Tmem175 Knockout cell line (H9C2)

Tmem175 Knockout cell line (H9C2) Order

Catalog#

YKO-R061

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

Tmem175 Knockout cell line (H9C2)

Species

Gene Name

Tmem175

Gene ID

305623

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Target region

Knockout region: Exon 2 (based on transcript Tmem175-201)
Validation: PCR+Sanger Sequencing

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the Tmem175 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the H9C2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, Tmem175 gene knockout Tmem175 Knockout cell line (H9C2) will be expanded and cryopreserved.

Knockout Cell Line Work Flow and Validatio

Related Popular Products

Mouse Genotyping Kit

High-performance Mouse Genotyping Kit
More details Order

Catalog#: YK-MGH-50;YK-MGH-100;YK-MGH-250

Size: ~50 tests;~250 tests;~250 tests

Instruction:

EZ-cryo™ cell freezing medium

EZ-cryo™ Cell Freezing Medium
More details Order

Catalog#: YK-CR-50;YK-CR-100

Size: 50ml;100ml

Instruction:

KO Cell Lines

TMEM175 Knockout cell line （SH-SY5Y ）
inquiry More details

Organism: Human

Gene Name: TMEM175

Gene ID: 84286

Size: 1*10^6

Catalog#: YKO-H907

YKO-H907(SH-SY5Y)
TMEM175 Knockout cell line (U-2 OS)
inquiry More details

Organism: Human

Gene Name: TMEM175

Gene ID: 84286

Size: 1*10^6

Catalog#: YKO-H714

YKO-H714(U-2 OS)
TMEM175 Knockout cell line (Hela)
inquiry More details

Organism: Human

Gene Name: TMEM175

Gene ID: 84286

Size: 1*10^6

Catalog#: YKO-H935

YKO-H935(Hela)

Related Popular Products

Mouse Genotyping Kit

High-performance Mouse Genotyping Kit

Price (USD): -;-;-

Catalog#: YK-MGH-50;YK-MGH-100;YK-MGH-250

Size: ~50 tests;~250 tests;~250 tests

Instruction:

inquiry More details

EZ-cryo™ cell freezing medium

EZ-cryo™ Cell Freezing Medium

Price (USD): -;-

Catalog#: YK-CR-50;YK-CR-100

Size: 50ml;100ml

Instruction:

inquiry More details

KO Cell Lines

TMEM175 Knockout cell line （SH-SY5Y ）

Organism: Human

Gene Name: TMEM175

Gene ID: 84286

Size: 1*10^6

Catalog#: YKO-H907

inquiry More details
TMEM175 Knockout cell line (U-2 OS)

Organism: Human

Gene Name: TMEM175

Gene ID: 84286

Size: 1*10^6

Catalog#: YKO-H714

inquiry More details
TMEM175 Knockout cell line (Hela)

Organism: Human

Gene Name: TMEM175

Gene ID: 84286

Size: 1*10^6

Catalog#: YKO-H935

inquiry More details

Tmem175 Knockout cell line (H9C2)

Ubigene